Greiner Bio-One

Greiner Bio-One International GmbH is an international medical technology company made up of four business divisions.

Preanalytics develops innovative collection systems for human and veterinary samples that make the flow of daily routine tasks in hospitals, laboratories or doctors’ surgeries simpler and safer.

BioScience is a technology partner to universities and research organizations, as well as to the diagnostics and pharmaceutical industries. BioScience is a leading supplier of specialist products for the analysis of cell cultures and microplates.

Diagnostics specializes in the development of molecular-biological analysis methods. The field of DNA array applications ranges from human diagnostics to food technology.

As an Original Equipment Manufacturer (OEM), Greiner Bio-One provides solutions in the area of custom-made design developments and production processes for the life sciences and medical sectors.

For more details, please visit: https://www.gbo.com/en

Preanalytics

Venous Blood Collection

The VACUETTE® brand name has been synonymous with progress and innovation in blood collection for many years. Just like many other trend-setters, the VACUETTE® system has often been copied too, but its quality and safety have never been equalled.

All of the components of the VACUETTE® system are available in different versions covering a wide spectrum of applications. The range of tubes covers all requirements of modern routine laboratory practice, as well as specialist laboratory needs. The tube holders and puncture systems can be selected in accordance with the patient’s individual vein conditions and based on various safety requirements.

Capillary Blood Collection

MiniCollect® Capillary Blood Collection System. For your most precious samples.

MiniCollect® offers a gentle way to collect small blood samples for a wide range of analyses. The system is perfectly suited for young children, geriatric patients as well as patients with fragile veins. MiniCollect® is also recommended in situations where venous blood collection proves particularly difficult, such as for patients with extensive burns.

MiniCollect® is the ideal system for collecting, transporting and processing capillary blood.

PrUrine Collection

The Capto media platform has been developed to allow efficient and economical handling of large volumes of feed.

Safety Products

In the healthcare sector sharps injuries account for most work-related transmissions of infectious diseases, whereby needlestick injuries present by far the greatest risk. More than 50% of all registered cases concern nursing staff, followed by doctors and laboratory staff.
However, needlestick injuries need not be an occupational risk. By using VACUETTE® Safety Products, this danger can be virtually eliminated.
Furthermore, by working with VACUETTE® Safety products you are compliant with criteria given in the EU Directive (EU 2010/32).

Accessories

Simple handling and safety are of the highest importance during daily routine procedures. The requirements of the user are therefore the prime consideration during development of VACUETTE® Blood Collection Accessories.

Disposal

Blood collection accessories, as well as all other pointed and sharp objects, must be disposed of in sharps-proof boxes. All Sharps Disposal Container from Greiner Bio-One have a large opening for the disposal of sharp and pointed objects. The sizes available range from a fill volume of 0.6 litres to up to 50 litres.

Preanalytics Catalogue

BioScience

Cell Culture Products

Greiner Bio-One GmbH develops and distributes products for the cultivation, storage and separation of cells and for specific applications in medical, pharmaceutical and biotechnological research. The company’s product line CELLSTAR® offers a wide spectrum of cell culture flasks, dishes and plates. Their functional design, particular material properties and special coatings enable these vessels to be used not only in standard cell culture but also in cancer research, viral diagnostics, genetic engineering and vaccine production.
In response to the increasing automation of cell culture procedures, Greiner Bio-One developed the AutoFlask™, a cell culture flask for automated systems. With the ThinCert™Plate and appropriate cell culture inserts, Greiner Bio-One has also created an innovative platform for compatibility tests using skin models in the chemical and cosmetic industries.
The Cell Culture product sector was recently expanded with the innovative Advanced TC™ polymer modification. These cell culture products have a special surface that enables more effective cultivation of fastidious cells and facilitates complex testing systems.

3D Cell Culture

The core technology of Greiner Bio-One’s Magnetic 3D Cell Culture is the magnetization of cells with biocompatible NanoShuttle™-PL. The reproducible formation of one spheroid per well in an F-bottom plate with Cell-Repellent surface is forced by magnets either by levitation or bioprinting, to form structurally and biologically representative 3D models in-vitro

Mass Cell Culture - CELLdisc™

CELLdisc™ is a new multilayer device covering a range of cell culture surfaces from 1,000 cm² up to 10,000 cm². The innovative ergonomic design of CELLdisc™ provides a versatile system for the propagation of adherent mammalian cells from research scale to industrial batches. In addition, a centrally located channel allows an uniform distribution of gas throughout the device. A socle-rim guaranties that the bottom layer of the CELLdisc™ does not touch the surface of the incubator. The compact and robust cylindrical device is ideally suited for automation and upscaling of mass cell culture.

Microplates

For decades, Greiner Bio-One GmbH has been developing and producing plastic microplates that are used throughout the world in laboratories for diagnostics and research. Microplates for high-throughput screening (HTS) enable fast, automated analysis of a multitude of substances. This high level of efficiency ensures successful development of new drugs, for example.

With one of the world’s largest product ranges, Greiner Bio-One is a leading supplier of microplates. Besides microplates, used for drug screening, the company also offers special plates for immunology, protein crystallisation, DNA analysis and microsystem technology.

Greiner Bio-One is one of the innovation leaders in the sector. In a number of cases, new developments are carried out in close collaboration with customers such as Boehringer Ingelheim Pharma GmbH & Co. KG and Novartis AG, which benefit from the company’s decades of experience with various materials and surface modifications.

One of the latest developments in this product sector are non-binding microplates. By repelling protein, these surfaces optimise the biochemical screening for active substances, as the influence of the plate surface on the test result is reduced considerably.

Immunology / HLA

ELISA (Enzyme-Linked Immunosorbent Assay) is probably the most widely used biochemical method in laboratory analysis and diagnostics. Analytes such as peptides, proteins, antibodies and hormones can be detected selectively in low concentrations among a multitude of other substances and be quantified. Additionally, ELISAs are rapid, sensitive, cost-effective and can be performed in a high-throughput manner. ELISA is used in a variety of different assay types (e.g. direct ELISA, indirect ELISA, sandwich ELISA, competitive ELISA). Nevertheless, all ELISA variants are based on the same principle the binding of one assay component – antigen or specific antibody – to a solid surface and the selective interaction between both assay components. Molecules not specifically interacting with the assay component bound to the solid surface are washed away during the assay. For detection of the interaction the antibody or antigen is labelled or linked to an enzyme (direct ELISA). Alternatively, a secondary antibody conjugate can be used (indirect ELISA). The assay is processed by adding an enzymatic substrate to produce a measurable signal (colorimetric, fluorescent or luminescent). The strength of the signal indicates the quantity of analytes in the sample.

Microbiology / Bacteriology

Petri Dishes

Available in different sizes
Easy stacking
Vented for better gas exchange and non vented for long incubation period
Heavy design for better heat resistance using hot agar
Irradiated options are also available on request
Manufactured from crystal clear polystyrene for optimal analysis by microscopy

Tubes / Beakers

Tubes are made of the following materials:

Polystyrene: Is ideally suited for optical measurements as a result of its high clarity.
Polypropylene: Displays high thermal, mechanical and chemical resistance. It is therefore recommended for the storage of chemical and biological samples.
Polyethylene: Is characterised by high thermal and chemical resistance.

The range of tubes available:

with round or conical bottom
with or without skirt
in sterile or non-sterile version

In addition, it is possible to order appropriate closures in the form of grip stoppers and screw caps.
The product range is completed by tubes with a two-position vent stopper. This special stopper enables ventilation or an airtight closure of the tube, depending on the position of the stopper.

Please refer to the Technical Appendix for information on the max. relative centrifugal force (RCF) and chemical and thermal resistance of our tubes.

Liquid Handling

Serological Pipettes

Increased volume from negative graduations
Pipette colour code according to international standards
Maximum clarity from high-grade polystyrene
Lot number and expiry date on each bag
All pipettes are supplied with a filter for protection against the suction of liquid into the pipetting device
Short-format pipettes (shorties) permit back-saving work

Pipette Tips
Pipette tips are a key component in day-to-day life science research and need to be of a high quality to ensure confidence and consistency in pipetting results. Our tips are manufactured from high-grade polypropylene to give the optimum in performance and fit. Additionally, polypropylene has a high breaking strength, is dimensionally stable, heat-resistant up to approx. +140 °C, i.e. is autoclavable, and cold-resistant down to -190 °C. All coloured Greiner Bio-One pipette tips are manufactured exclusively with heavy metal free dyes.
Pipette tips from Greiner Bio-One are therefore free of heavy metals.

Sapphire Pipette Tips
The Sapphire product family comprises standard, filter and low retention tips manufactured from virgin materials. All tips are transparent, graduated, and allow precise and comfortable pipetting with maximal recovery.

Molecular Biology

PCR Tubes

The PCR range has a product for all applications, including 0.2 ml and 0.5 ml tubes with flat caps, PCR tube strips with 8 tubes or as individual reaction tubes, as well as PCR microplates. The thin wall construction optimises the heat transfer from the block to the reaction solution.

PCR Microplates

The use of the 96 well format allows the scale up of basic PCR work, while the 384 well format is ideal for high-throughput screening projects. All microplates are made of thin-walled polypropylene. This optimises the heat transfer from the thermoblock to the reaction solution. Our heat-resistant sealers AMPLIseal™, VIEWseal™ and SILVERseal™ are ideal for sealing the microplates during PCR, and the 96 well microplate may also be sealed with.

Protein Crystallisation

An important method for the determination of protein structures is x-ray analysis of protein crystals. The determination of the three-dimensional structure of proteins has contributed towards major advances in basic research, particularly in the fields of structural genomics and structure-based drug design. The most commonly used method for the crystallization of proteins is vapour diffusion which comprises both the sitting drop and hanging drop methods . One drop of protein solution is mixed with one drop of reagent solution and incubated together with a larger volume of reagent solution in a sealed well. Concentration gradients between the sample drop and the reservoir solution are balanced out by diffusion, which induces the crystallisation process if the correct conditions have been selected.
The microbatch method in which the sample drop is covered with oil is also widely used, and in this technique the choice of oil determines the rate of diffusion of the water in the sample drop through the oil. Numerous factors affect the crystallisation of proteins. Since the optimal crystallisation conditions generally cannot be predicted, a large number of attempts is often necessary in order to determine and optimise the appropriate conditions. Protein crystallisation therefore still represents a major bottleneck in structure analysis. The use of high-throughput technologies, such as pipetting robots and standardised microplates, makes it possible to test a large number of crystallization conditions in a short period of time and with relatively small amounts of protein.
The CrystalStar™ product range from Greiner Bio-One is a family of crystallisation plates and accessories designed specifically for high-throughput crystallization.

Seperation

Different separation techniques can be used to enrich certain particles (DNA, RNA, proteins, organelles, vesicles, micelles, cells etc.) specifically from complex biological mixtures such as cell and tissue homogenates, blood, urine and other body fluids, so that they can then be selectively investigated. Separation of these types of particles can be based either on the different sedimentation rates of different particles in a fluid, or on their different densities. Density gradient centrifugation (also referred to as band, equilibrium or isopycnic centrifugation), exploits the principle that particles of a certain density migrate into a density gradient until they reach an equilibrium density layer. The first applications of density gradient centrifugation were reported in the early 1950s. Back then, cell organelles were enriched with the aid of buffered saccharose gradients and it is uncontested that the knowledge gained with these enriched materials made a contribution to modern molecular biology. Soon it was discovered that the enrichment of mammalian cells requires more complex separation media, particularly due to their sensitivity towards osmotic fluctuation. Noble and Boyum described methods for separating mononuclear cells from whole blood and bone marrow as early as 1967 and 1968. Based on this pioneering scientific work, numerous applications in today‘s biomedical research and routine diagnostics require highly enriched, viable and functionally intact cell populations as the starting material. The separation of such cells by density gradient centrifugation has proven to be the most often used method due to its uncomplicated and robust nature.

With Leucosep™, Greiner Bio-One optimised density gradient centrifugation whilst making it user-friendly. Alongside this, OncoQuick® was developed to extend the spectrum of applications to deal specifically with oncological targets.

Cryo.s™ and Biobanking Tubes

With the brand Cryo.s™ Greiner Bio-One combines more than 30 years of experience in the manufacturing of cryogenic tubes with latest technology and innovation. Cryo.s™ may be used in combination with a variety of accessory boxes and racks for storing biological samples at ultra-low temperatures (e.g. in gas phase of liquid nitrogen). All tubes are guaranteed free of endotoxins, DNA, Dnase, Rnase; only virgin, medical grade, USP class VI certified, leachable-free polypropylene is used for the manufacturing of Cryo.s™.

Lids / Sealers / CapMats

Reaction Tubes / Analyser Cups

Accessories

BioScience Catalogue

Diagnostics

Greiner Bio-One Diagnostics specialises in the development of new molecular biology analysis methods. The field of application of ‘DNA arrays’ as a key technology ranges from human diagnostics to food technology. They can be used to uniquely identify bacteria or viruses via the specific genetic fingerprint and make a diagnosis quickly and safely, for example.